Chris Ashton has been ruled out of England's match against Australia on Saturday with a calf injury.
Ashton came off the bench to make his first England outing in four years in the victory over South Africa at the beginning of the month, before starting the New Zealand and Japan fixtures and scoring a try against the All Blacks.
But the Sale Sharks wing was replaced due to injury against the Brave Blossoms and is now set to miss two weeks of action.
"Chris came off with an injury on Saturday," said forwards coach Steve Borthwick. "We investigated the injury and unfortunately he's going to miss a couple of weeks, which means he's clearly unavailable for our game this weekend. He'll return home later today.
"It's only a couple of weeks. It's frustrating. I'm disappointed for him, disappointed for us.
"It's great the work he's done in camp over the last few weeks but hopefully he'll be back on the field very soon."
Borthwick gave the squad an otherwise clean bill of health, with Joe Cokanasiga, who scored a try on debut against Japan, working towards full involvement in training.
"Joe Cokanasiga didn't train with the team today, he trained on his own programme, which is just part of the individual management that we have," added Borthwick.
"But he's fully fit and available for the weekend."
Nathan Hughes is back in the squad following the completion of a six-week ban for punching Gloucester flanker Lewis Ludlow and questioning his punishment on social media.
"Nathan's done well since coming back into camp," said Borthwick. "He trained on Friday, trained again Sunday and trained with the team the last couple of days, doing really well. He's in good shape.
"I think he's worked really hard on his conditioning. Clearly you've got to prepare as well as you possibly can in those circumstances.
"You actually make improvements in lots of areas when you have that opportunity to train like that. And I think Nathan's worked really hard in this period he's had off."
12 de diciembre 2018
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|Tuchel hails magnificent PSG as Red Star win seals last-16 spot|
|Mbappe admits frank discussions inspired PSG´s Champions League progress|
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11 de diciembre 2018
|Barcelona equal Champions League record|
|Composed and controlled Liverpool see off Napoli to progress|
|Tenerife back to winning ways, AEK beat Hapoel|
|Howedes ´hurt´ after emotional Schalke return ends in defeat|
|Kane ´buzzing´ over ´not bad´ draw at Barcelona|
|Robertson hails Alisson´s ´unbelievable´ save as Liverpool progress|
|Red Star Belgrade 1 Paris Saint-Germain 4: Neymar brilliance helps secure Group C top spot|
|Inter 1 PSV 1: Icardi header not enough for the Nerazzurri|
|Liverpool 1 Napoli 0: Superb Salah sends Reds through|
|Club Brugge 0 Atletico Madrid 0: Simeone´s men miss out on top spot|
|Barcelona 1 Tottenham 1: Lucas rescues Spurs late on to secure progress|
|Monaco 0 Borussia Dortmund 2: Guerreiro double snatches top spot from Atletico|
|Butler´s groin strain ´nothing significant´ – 76ers coach Brown|
|Woakes, Curran among leading lights at IPL auction|
|Blue Jays release five-time All-Star Tulowitzki|
|Mourinho: Professionals care more than the fans|
|Casillas reaches 100 Champions League wins|
|Expansion franchise Cincinnati sign Garza, send Kamara to Rapids|
|Manchester City sign United States goalkeeper Steffen for reported £7m|
|Schalke 1 Lokomotiv Moscow 0: Russians miss chance for Europa League reprieve|
|Galatasaray 2 Porto 3: Terim´s men into Europa League after penalty drama|
|Jackman leaves Dragons role|
|Tottenham boosted as Barcelona bench Messi|
|Mane and Henderson in Liverpool XI to face Napoli|
|Mourinho shoots down question over how to get the best from Pogba|
|Ronaldo to start as Allegri aims to avoid Champions League big guns|
|Odriozola: I´m in love with Benzema, he´s the best number nine in the world|
|Vikings fire OC DeFilippo after loss to Seahawks|
|Marcelino suggests Valencia open to Batshuayi departure|
|Bottas to make rally debut in title-winning M-Sport Ford Fiesta|
|Kovac dismisses Pavard speculation|
|Yaya Toure leaves Olympiacos after just five appearances|
|Riise challenges Liverpool to banish Champions League disappointment against Napoli|
|We rallied around it - Durant on Green confrontation aftermath|
|Valencia ´p****d off´ with Fellaini´s controversial Young Boys winner - Soler|
|More must be done to combat racism at Premier League grounds – Chalobah|
|Napoli will not sack ´big signing´ Ancelotti if they miss Champions League qualification, says De La|
|Guardiola not interested in ´exceptional´ Saul|
|Shai gives Windies hope with thrilling ODI run chase|
|Ten Hag unsure whether to risk Ajax stars against Bayern|
|Erasmus to work with Bulls as Springboks assist Super Rugby sides|
|Paul Gascoigne pleads not guilty to sexual assault|
|Joshua is ´begging´ for unification fight, claims Wilder|
|Silva, Fernandinho add to Guardiola´s injury woes|
|Hosszu storms to 14th gold, Seto smashes Le Clos´ world record|
|Raheem is an incredible human being - Guardiola rallies behind City star Sterling|
|Sexton pens IRFU extension until 2021|
|Ajax boss Ten Hag can do big things, says Bayern chief Rummenigge|
|He´s good at basketball – Kerr running out of superlatives for Curry|
|Green thrilled to make Warriors return in victory over Timberwolves|
|Madrid squad great and humble - Solari responds to Ronaldo|
|Langer unsure what to expect from Perth wicket|
|Fournette subjected to alleged racial abuse in Titans loss - Marrone|
|Better and better - Mbappe hails Neymar link|
|It´s the end of one of the best movies you could ever see - James pays tribute to retiring Wade|
|NASA invites Curry to see moon rocks|
|No plan B for LaLiga Miami game after Barcelona withdrawal|
|Messi´s English haul, Neymar eyes hat-trick record - Champions League in Opta numbers|
|LeBron trumps Wade in last dance, Curry scores 38 points|
|Seahawks secure dominant win over Vikings|
|Iniesta visits Messi and former club Barcelona|
|Drake makes plea for ball thrown into stands after Miami miracle|
|´Toughest pretty boy I´ve ever met´ - Langer dismisses Paine concerns|
|Van Dijk says Liverpool can challenge for double ahead of Napoli decider|